Composite

Part:BBa_K1433002

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)
Revision as of 11:05, 16 October 2014 by Lilina (Talk | contribs)

attL-J23110-attR

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state.

File:ZJU int xis bplr reverse

composition:

    This part is composed of 2 elements.:
  1. Promoter (reverse): BBa_J23110, a middle-ground Bacterial constitutive promoter.
  2. attL and attR sites: Recognition sites for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.

This part promotes the transcription and translation of the upstream genes. When it is treated with both of Bxb1 gp35 and gp47 at certain ratio, downstream gene will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 74
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24
    Illegal BsaI.rc site found at 107


[edit]
Categories
Parameters
None