Reporter
BFP

Part:BBa_K1381025

Designed by: Viktor Blomkvist, Tim Hagelby Edstrom, Laura Pacoste and Tersea Reinli   Group: iGEM14_Uppsala   (2014-10-07)
Revision as of 10:51, 16 October 2014 by StephyHe (Talk | contribs)

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B0034-BFP (RFC25)

Used for N-terminal-BFP fusion protein using Freiburg standard. Contains an RBS and ATG before the NgoMIV site so can be expressed separetely if needed.

This part is a mutagenise of BBa_K592024 (B0034-BFP) to make it compatible with RFC25. It was designed to get an ATG followed by an NgoMIV site upstream of the BFP CDS. The original ATG from the BFP CDS was removed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None