Tag
LAA

Part:BBa_M0050:Experience

Designed by: Felix Moser   Group: Endy Lab   (2007-12-05)
Revision as of 09:19, 16 October 2014 by AStikane (Talk | contribs) (→‎User Reviews)


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Applications of BBa_M0050

User Reviews

UNIQe04a6f9cee9488c9-partinfo-00000000-QINU UNIQe04a6f9cee9488c9-partinfo-00000001-QINU


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iGEM14_Edinburgh

iGEM14_Edinburgh added this tag to RFP (Part:BBa_K1399002) and GFP (Part:BBa_K1399006) and constructed lactose/IPTG inducible measurement pathway (Part:BBa_K1399012 and Part:BBa_K1399016, respectively) to characterize and compare the instability of this SsrA-LAA tagged RFP and GFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)

SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).
Summary of BioBricks to which our characterization data is applicable.
SsrA degron tagged GFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).
Summary of BioBricks to which our characterization data is applicable.