Composite

Part:BBa_K1463002

Designed by: iGEM14_Glasgow   Group: iGEM14_Glasgow   (2014-10-15)
Revision as of 08:30, 16 October 2014 by Scolloms (Talk | contribs)

Recombinase Switch With GFP (weaker RBS)

PhiC31-based recombinase switch with GFP to the right and a leftward facing promoter. PhiC31 integrase switches on GFP expression. GFP contains a weaker ribosome binding site than in BBa_K1463001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 105
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 938


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Parameters
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