Coding

Part:BBa_K1321014

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-15)
Revision as of 02:01, 16 October 2014 by Registry (Talk | contribs)

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CBDCipA with N and C-terminal linker

This CBD is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus (UniProt ID Q06851 link http://www.uniprot.org/uniprot/Q06851). It is in RFC25 format to allow for easy use in protein fusions.

The CBD is part of the CBM3 family (link to cazy http://www.cazy.org/CBM3.html). This CBD has been used in many application: in fusions with cell adhesion peptides to enhance the properties of cellulose as a cell-growth matrix (Andrade et al 2010a, Andrade et al 2010b), fused to enzymes to remove contaminants from water (Kauffmann et al 2000) and fused to an antimicrobial peptide (Ramos, Domingues & Gama 2010).

At present the cloning for these constructs is still in progress to correct an illegal EcoRI site which was identified in the parts with this CBD. This can be achieved with a silent mutation via site-directed mutagenesis and we aim to send these parts to the registry once this is complete.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 148
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 148
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 148
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/cellulose
Parameters
None