Composite

Part:BBa_K1462090:Design

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-10)
Revision as of 01:46, 16 October 2014 by Weapon (Talk | contribs) (→‎Design Notes)

Gal1+cox6+GFP+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1325


Design Notes

　To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.

Source

COXVI is obtained from genome of saccharomyces cerevisiae, the other parts is from the kit provided by official

References