Regulatory
C2

Part:BBa_K1442033

Designed by: Yin Ho Vong, Iva Burova   Group: iGEM14_Warwick   (2014-09-05)
Revision as of 01:17, 16 October 2014 by IvaBurova (Talk | contribs)

C2 3

Each unique RNA dependent RNA polymerase (RdRP) initiates de novo replication of a RNA strand by interacting with RdRP-specific RNA sequences, henceforth called RdRP/RNA promoters. Please refer to Section: RdRP function for further details. The RdRP chosen for our project is taken from the Hepatitis C virus (HCV). The C2 RNA promoter was identified as such by Heinz, Kao (2000) , along with a number of other sequences. All of them possess a few common characteristics: an initiation cytidylate at the 3’ end, where the replication starts; and a stable secondary structure- single stranded tail and a stem of various length. Replication stops at the 5’ end.

Usage

Used as an RNA promoter to direct replication by RdRP.


Mathematical Modelling

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 38
    Illegal NgoMIV site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aC2 3'UTR Promoter for RdRP