Generator

Part:BBa_K1444018:Design

Designed by: Dave Curran   Group: iGEM14_Calgary   (2014-10-15)
Revision as of 00:04, 16 October 2014 by DCurran (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Xylose -> comK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 557
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 170
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 557
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 557
    Illegal EcoRI site found at 572
    Illegal BamHI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 557
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 170
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 557
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 170
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 743


Design Notes

None.


Source

This part was amplified from the chromosome of a strain of B. subtilis derived from that described by Zhang & Zhang (2010). The strain was generously given to us by [http://www.bio.ucalgary.ca/contact/faculty/wong.html Dr. S.L. Wong], who routinely uses it in his synthetic biology research on B. subtilis at the University of Calgary.

References