Part:BBa_I11012:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ1a5dd3482a24a52c-partinfo-00000000-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ1a5dd3482a24a52c-partinfo-00000002-QINU

•• Edinburgh IGEM 2014

iGEM14_Edinburgh added this tag to RFP (Part:BBa_K1399000) and constructed lactose/IPTG inducible measurement pathway (Part:BBa_K1399010) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].) SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Summary of BioBricks to which our characterization data is applicable.

;