Regulatory
R5

Part:BBa_K1442103

Designed by: Dan Goss   Group: iGEM14_Warwick   (2014-10-07)
Revision as of 22:30, 15 October 2014 by CarriedC (Talk | contribs)

Reverse 5

Reverse sense 5'UTR region of the Hepatitis C Virus genome strain 1b isolate Con1.

This was used as a promoter for the positive to negative sense transcription of the RNA strand by RNA dependent RNA polymerase coded by NS5B gene in HCV genome

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 142
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 397
    Illegal NgoMIV site found at 426
    Illegal AgeI site found at 236
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 69


[edit]
Categories
Parameters
n/aReverse 5UTR