Part:BBa_K1475002

FAT-2 delta 12 desaturase from C. elegans

This part encodes the FAT-2 delta 12 desaturase from Caenorhabditis elegans and uses oleic acid (C18:1Δ9) as a substrate to produce linoleic acid (C18:2∆9,12).

Usage and Biology

Fatty acyl desaturases act on acyl groups to introduce double bonds. Fat-2 is a ∆12 fatty acyl desaturase which is able to introduce a double bond in C16 and C18 fatty acids in Caenorhabditis elegans, and it has been shown to work as a ∆15 desaturase in yeast using oleic acid (C18:1∆9) as a substrate [1]. In nature the desaturation of fatty acids is important for keeping the cell membrane fluid in cold conditions [2].

This part contains only the coding region of the fat-2 ∆12-desaturase from “Caenorhabditis elegans”, the natural gene consists of three exons. [2]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 117
  Illegal BamHI site found at 253
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 169
  Illegal SapI site found at 582
  

References

1. Xue-Rong Zhou, et al.: Caenorhabditis elegans Δ12-Desaturase FAT-2 Is a Bifunctional Desaturase Able to Desaturate a Diverse Range of Fatty Acid Substrates at the Δ12 and Δ15 Positions. J Biol Chem. Dec 23, 2011; 286(51): p. 43644–43650, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243535/
2. Wormbase, 10 Jun 2010: fat-2. http://www.wormbase.org/species/c_elegans/gene/WBGene00001394?query=fat-2#0-9e-3.
3. Uniprot, fat-2_CAEEL accession number: G5EGA5. http://www.uniprot.org/uniprot/G5EGA5