Coding

Part:BBa_K1475003:Design

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-10-05)
Revision as of 20:31, 15 October 2014 by Danie12 (Talk | contribs)

tetracyclin repressor from transposon Tn10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was constructed by PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040) using the following primers.
Forward primer:
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
Reverse primer:
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC



Source

Organizm: Escherichia coli


References