Coding
Part:BBa_K1475003:Design
Designed by: Daniel Weltz Pedersen Group: iGEM14_SDU-Denmark (2014-10-05)
tetracyclin repressor from transposon Tn10
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was constructed by PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040) using the following primers:
Forward primer:
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
Reverse primer:
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC
Source
This part was constructed from the tetracycline repressor from transposon Tn10 (+LVA) (Part:BBa_C0040) using primers to remove the LVA protein digestion tag. That is THIS PART DOES NOT CONTAIN THE LVA PROTEIN DIGESTION TAG.