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Part:BBa_K1554000

Designed by: Valencia_UPV team members   Group: iGEM14_Valencia_UPV   (2014-09-26)
Revision as of 18:47, 15 October 2014 by Lestelles (Talk | contribs)

TA29 promoter

The TA29 promoter is an anther-specific promoter. One of the most used strategies of biosafety in plants in male sterility. A cytotoxic gene (like barnase, ipt, RIP...) under the control of this promoter leads to a plant without fertile pollen. Our team used it in the construction of Part:BBa_K1554004 and Part:BBa_K1554005

Usage and Biology

The TA29 promoter permits the specific expression of genes under its regulation in the tapetum, a layer found in the anthers of flowering plants. One of the most used strategies related to biosafety in plants consists in producing male sterility. A cytotoxic gene (like barnase, ipt, RIP...) its control leads to a plant without fertile pollen, being unable to be self-pollinated and hence, unable to spread in an uncontrolled way in the field. Our team used it in the construction of Part:BBa_K1554004 and Part:BBa_K1554005, two biosafety modules for plants submitted to the Registry of Parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//chassis/eukaryote/nbenthamiana
//promoter
Parameters
None