Part:BBa_K1342001

zinTp_AspA

ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion. We assembled ZinTp(BBa_K1342005), RBS(BBa_B0034), ApeA(BBa_K1342002), Dter(BBa_B0015).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 179
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1071
  Illegal AgeI site found at 600
  Illegal AgeI site found at 1452
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1111
  

TLC Assay

Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)