Part:BBa_K1316013

pRhamnose regulating csgB + J23110 constitutive Anderson promoter regulating csgA

This part is meant to constitutively express the curli gene csgB which codes for the production of the curli protein that is anchored to the cell membrane (CsgB). The csgA gene codes for the major curli protein (CsgA), which forms long fibrils with other CsgA monomers in a self-assembly process. These CsgA long fibrils bind to the anchored CsgB protein forming the amyloid-like curli structure. As many more CsgA than CsgB subunits are required for curli formation, this part intends to have an already high amount of CsgA present in the cell (constitutively expressed) and, once the cell is induced (with Rhamnose in this case), only CsgB protein is required to be expressed for curli formation, thus leading to a faster curli generation process.

Characterization

Different type of experiments were conducted to characterize this BioBrick:

Plate Reader

A plate reader was used to detect cell concentration (OD600) and fluorescence of cells carrying the curli-forming BioBricks (BBa_K1316013-BBa_K1316015), which were at the same time also carrying the BBa_K1316016 construct, constitutively expressing eGFP. By doing this assay, cells were observed to be more attached to the walls of a 96-well plate when they had curli-forming BioBricks induced with Rhamnose (cruli genes induced) (figure 1).

Figure 1: OD after washing out the cells twice as a fraction of initial OD observed on 96-well plates, with (+) and without (-) induction of the curli-formation genes. Induced cells are induced with 1% rhamnose solution.

Confocal Microscopy

Confocal microscpoy technology was used to observe the deposition of cells at the bottom of the microscope slide. Figures 2 and 3 show how after induction with Rhamnose the cells forming curli are attached faster to the surface (bottom) of the microscope slide than when they are not induced.

Figure 2: Fluorescent images taken using the Confocal Microscope of the cells carrying the CC constructs BBa_K1316013 and BBa_K1316016, induced (left) and non-induced (right).

For more information about the characterisation of this construct visit the Conductive Curli module characterisation on our wiki page! (http://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 516
  Illegal NheI site found at 551
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]