Coding

Part:BBa_K1469008:Design

Designed by: Philip Hartz   Group: iGEM14_Saarland   (2014-10-02)
Revision as of 14:06, 15 October 2014 by Grafzahl (Talk | contribs) (→‎Design Notes)

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glmM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 920


Design Notes

Endogenous XbaI restriction site is still present.[http://2014.igem.org/Team:Saarland/4_step#BioBrick]

Source

Amplification of genomic DNA of Bacillus megaterium strain MS941.

References