Coding

Part:BBa_K1469008:Design

Designed by: Philip Hartz   Group: iGEM14_Saarland   (2014-10-02)
Revision as of 13:59, 15 October 2014 by Grafzahl (Talk | contribs) (→‎Design Notes)


glmM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 920


Design Notes

Endogenous XbaI restriction site is still present.

Source

Amplification of genomic DNA of BAcillus megaterium strain MS941

References