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Part:BBa_K1463000

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-09-09)
Revision as of 12:52, 15 October 2014 by Scolloms (Talk | contribs)

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.
PhiC31 integrase flips the orientation of the DNA between attP and attB, changing the direction of the promoter, switching rfp off and gfp on.

Switch_diagram_sites_small2.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 813
    Illegal NheI site found at 836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1668


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