Regulatory

Part:BBa_K1316004

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-08-24)
Revision as of 12:05, 15 October 2014 by Joanctd.igem (Talk | contribs)

ybiJ promoter

Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.

The functionality of the ybiJ promoter is proven by the production of mKate2 in response to 2,4-DNT on the characterisation of BioBrick BBa_K1316005 (https://parts.igem.org/Part:BBa_K1316005). Its response in front of 2,4-DNT, nevertheless, is much lower than the one of the yqjF promoter (BioBrick BBa_K1316003).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 381
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None