Reporter

Part:BBa_K1316001

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-08-24)
Revision as of 11:42, 15 October 2014 by Joanctd.igem (Talk | contribs)

mKate2 protein with double transcription terminator

mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. BBa_K823017 Double Terminator (B0012-B0011)

This Composite part was used for the construction of the final BioBricks BBa_K1316003, BBa_K1316005, BBa_K1316007, BBa_K1316008 and BBa_K1316009. The functionality of mKate2 is proven by the red fluorescence detected on the characterisation of BioBrick BBa_K1316003 (https://parts.igem.org/Part:BBa_K1316003). The advantage of adding the double terminator has not been proven because the constructs containing the N-genes (BBa_K1316007 and BBa_K1316008) and the construct having a combination of both ybiJ and yqjF promoters (BBa_K1316009) did not seem to be properly induced by the presence of 2,4-DNT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 454
    Illegal BsaI.rc site found at 643


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Categories
Parameters
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