Part:BBa_K1529301
Prhl(RL)-GFP
Prhl(RL) is a promoter that is activated by C4HSL.
We improved Prhl (BBa_R0071) by changing LuxR binding site of Plux (BBa_R0062) to RhlR binding site. (Fig.1 .)
The bottom structures of LuxR and RhlR are similar to each other, so RhlR can also bind to Lux Box. (Fig. 2.)
On the downstream of the promoter, GFP(BBa_J54103) is inserted as a reporter.
To characterize the function of the Prhl(RL) promoter (BBa_K1529300), we constructed this part, Prhl(RL)-GFP (BBa_K1529301), by inserting Prhl(RL) promoter upstream of a GFP coding sequence.
By using reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL.
We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay. (Fig. 3.)
For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 777
None |