Part:BBa_K1341001
PATH WAY TEST SYS IN BIO-COMPASS
This system has proved that we have successful insert the BBa_K1341000 in the testing path way (we designed a AFL III Restriction Enzyme cutting site in the No.5 node sequence, and we linked the matching AFL III Restriction Enzyme cutting site sequence on switch device's(BBa_K1341000)ends.).Besides, this system also have a BamH I Restriction Enzyme cutting site in the No.2(start) node's sequence, a Nco I Restriction Enzyme cutting site in the No.8(terminal） node's sequence. With the same method, the TetR device (BBa_K1341003) and LacI device (BBa_K1341002) can insert in this measurement system according to the requirement. By using the aTc and IPTG, we can prove whether the path way is the right result exactly such as this(2-4-5-7-8).
characterization
The analysis about the logic of the system
We can prove that the GFP signal part(BBa_K1341000) of the screen device has been insert in our path way test system((BBa_K1341000 is almost 1000bp, and the path way sequence is about 200 bp, this Electrophoresis result is about 1200bp), and we make this gene circuit(BBa_K1341001) do the follwing test about whether this path way circuit is the right way we want.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 11
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 180
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 837
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