Part:BBa_J100176:Experience

Purpose: We are testing if triclosan induces the FabHAF promoter in E.coli cells through fatty acid deprivation.

Method: We collected four E.coli colonies from the experimental plate, two green and two red. We also collected two green colonies from the positive control plate. We mixed these abstracted colonies with LB broth and ampicillin. We chose three of the six tubes prepared and added 20 µL of 0.5% triclosan from antimicrobial hand soap to one experimental, one negative, and one positive tube. The other three tubes do not have triclosan. We pipetted triplets of each sample then we used the Synergy machine to measure how much RFP was in each of the six tubes. We also measured the RFP of a blank well, which contained nothing but LBM media. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells. Then we generated our graph using the ratio of red fluorescence protein to cell density. Our graph illustrates duplicates of our samples rather than triplicates due to extreme outliers exhibited by the results from the Synergy machine.