Part:BBa_K1483003

Ssp GyrB Split Intein

N-Intein part of the ssp GyrB split intein. Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix. For immobilisation, parts need to be fused to the N-terminus of the N-intein. The C-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix.

The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (aquired by peptide synthesis and is thus not part of the registry)

This part can also be used in order to create fusion proteins, by attaching the C-Intein to a protein instead of the matrix. The part is therefore designed in RFC25.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]