Coding
aGal

Part:BBa_K1483002:Design

Designed by: Philipp H. O. Mayer and Philip Roessler   Group: iGEM14_Tuebingen   (2014-10-06)
Revision as of 19:52, 14 October 2014 by Philip (Talk | contribs)

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α-Galactosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 412


Design Notes

The part is designed in RFC25 in order to ease fusion with other parts, for example immobilisation tags.


Source

The protein encoded by this sequence naturally occurs in Bacteroides fragilis. This sequence was optimized for codon usage in E. coli K12 and obtained by in-vitro gene synthesis.

References

The amino acid sequence of the protein is based on data from a publication in [http://www.nature.com/nbt/journal/v25/n4/abs/nbt1298.html Nature biotechnology]

Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464.

The sequence can be found in [http://www.ncbi.nlm.nih.gov/protein/695442642?report=genbank&log$=protalign&blast_rank=1&RID=3UEFZXJE014 NCBI].

A patent claiming rights on this part can be found in the [http://appft1.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=/netahtml/PTO/srchnum.html&r=1&f=G&l=50&s1=20140220553.PGNR. United States Patent & Trademark Office]