Composite

Part:BBa_K1462520:Design

Designed by: zhanru Chen   Group: iGEM14_SCUT   (2014-10-10)
Revision as of 18:17, 14 October 2014 by Firedfish (Talk | contribs) (→‎Design Notes)

GAL1+YFP+SH3-lig+ADH1+GAL1+GFP+PDZ-lig+ADH1+GAL1+BFP+GBD-lig+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1755
    Illegal AgeI site found at 3300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2804


Design Notes

This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"[BBa_K1462490],"GAL1 + GFP + BFP ligand + ADH1"[BBa_K1462500] and GAL1 + YFP + SH3 ligand + ADH1[BBa_K1462510]. We planned to transmit this device to yeast with the different scale group of GBD,PDZ and SH3 scaffold protein.


Source

This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"[BBa_K1462490],"GAL1 + GFP + BFP ligand + ADH1"[BBa_K1462500] and GAL1 + YFP + SH3 ligand + ADH1[BBa_K1462510].

References

[1] John E Dueber, Gabriel C Wu, G Reza Malmirchegini, et al.Synthetic protein scaffolds provide modular control over metabolic flux.Nat Biotechnol. 2009 Aug;27(8):753-9.
[2] Schultz, J. et al. Specific interactions between the syntrophin PDZ domain and voltage-gated sodium channels. Nat Struct Biol 5, 19-24 (1998).
[3] Kim, A.S., Kakalis, L.T., Abdul-Manan, N., Liu, G.A. & Rosen, M.K. Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein. Nature 404, 151-158 (2000).
[4] Wu, X. et al. Structural basis for the specific interaction of lysine-containing proline-rich peptides with the N-terminal SH3 domain of c-Crk. Structure 3, 215-226 (1995).
[5] Harris, B.Z., Hillier, B.J. & Lim, W.A. Energetic determinants of internal motif recognition by PDZ domains. Biochemistry 40, 5921-5930 (2001).
[6] Dueber, J.E., Yeh, B.J., Chak, K. & Lim, W.A. Reprogramming control of an allosteric signaling switch through modular recombination. Science 301, 1904-1908 (2003).
[7] Nguyen, J.T., Turck, C.W., Cohen, F.E., Zuckermann, R.N. & Lim, W.A. Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors. Science 282, 2088-2092 (1998).