Generator

Part:BBa_K1381013

Designed by: Andries Boers, Eric Sandstrom, Kira Karlsson, Miranda Steirnborg and Jennifer Rosenius   Group: iGEM14_Uppsala   (2014-10-03)
Revision as of 17:22, 14 October 2014 by Ersa2517 (Talk | contribs)

J23100-B0034-CheZ

cheZ (BBa_K629003) combined with an RBS (BBa_B0034) and constitutive promoter (BBa_J23100). This part was created to see if expression of cheZ in a cheZ-knockout strain would restore its motility. Similar parts are: BBa_K1381011 and BBa_K1381012.

cheZ
Chemotaxis is the cell’s system for directed movement which allows the cell to move towards nutrition sources as well as away from toxic substances. The chemotactic system is a complex chain of phosphorylation reactions. At the end of the chain is the flagellum-binding protein CheY. The phosphorylated CheY (CheY-p) binds the flagella and causes the cell to tumble in place, essentially remaining immotile. The phosphatase CheZ dephosphorylates CheY, causing CheY to unbind to the flagella, resulting in directed movement of the cell [1].

References
[1] Madigan MT, Martinko JM, Stahl DA, Clark DP. 2010. Brock biology of microorganisms. 13th ed. Benjamin Cummings, San Francisco.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None