Part:BBa_K1342002

AspA LVA(-)

We removed LVA tag that renders protein susceptible to the action of tail-specific proteases from BBa_C0083 .
AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 72
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 964
  Illegal AgeI site found at 493
  Illegal AgeI site found at 1345
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1004
  

Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, addition cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)