Generator

Part:BBa_K1381011

Designed by: Andries Boers, Eric Sandstrom, Kira Karlsson, Miranda Steirnborg and Jennifer Rosenius   Group: iGEM14_Uppsala   (2014-10-03)
Revision as of 16:36, 14 October 2014 by Ersa2517 (Talk | contribs)

J23113-B0034-CheZ cheZ combined with an RBS and constitutive promoter.



cheZ
cheZ is a gene involved in the chemotactic pathway of E.coli. CheZ induces directed movement of the cell by dephosphorylating the flagella-binding protein CheY, causing CheY to unbind to the flagella. Thus, when cheZ is expressed, the cell will be motile and when cheZ is not expressed the cell will be immotile.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None