Generator

Part:BBa_K1486024:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-26)
Revision as of 16:36, 14 October 2014 by Ionepla (Talk | contribs)

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Yeast kanamycin resistance


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 166
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 166
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 166
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Taken out of a plasmid obtained via Addgene : Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan

Source

It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan.

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)

References