Part:BBa_K1486024

Yeast kanamycin resistance

Purpose of the Biobrick

This part encodes for the KanMx resistance gene and confers dominant selection of geneticin to yeast. It consists of a geneticin resistance, a constitutive TEF promoter and a constitutive TEF terminator. We fused it to our constructs to select the transformants after homologous recombinations.

Associated Biobricks

This part was received with the terminator ADH1 and we added it to all our constructs being fused with the pbs2 gene.

• yeast-sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026)
• RLuc-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486027)
• yeast-sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029)
• RLuc_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486030)

References

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 166
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 166
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 166
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 166
  
• 1000
  COMPATIBLE WITH RFC[1000]