Coding
aGal

Part:BBa_K1483002:Design

Designed by: Philipp H. O. Mayer and Philip Roessler   Group: iGEM14_Tuebingen   (2014-10-06)
Revision as of 15:50, 14 October 2014 by Philip (Talk | contribs) (→‎References)


α-Galactosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 412


Design Notes

Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.


Source

The protein encoded by this sequence naturally occurs in Bacteroides fragilis. This sequence was optimized for codon usage in E. coli K12 and synthesized.

References

The amino acid sequence of the protein is based on data from a publication in [http://www.nature.com/nbt/journal/v25/n4/abs/nbt1298.html Nature biotechnology]

Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464.

The sequence can be found in [http://www.ncbi.nlm.nih.gov/protein/695442642?report=genbank&log$=protalign&blast_rank=1&RID=3UEFZXJE014 NCBI].