Coding
NAGA

Part:BBa_K1483000:Design

Designed by: Philipp H.O. Mayer and Philip Roessler   Group: iGEM14_Tuebingen   (2014-10-06)
Revision as of 14:51, 14 October 2014 by Philip (Talk | contribs) (→‎References)

α-N-Acetylgalactosamindase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 405


Design Notes

Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags. Codon usage was modified for optimal expression in E. coli K12 and common restriction sites were removed.

Source

The protein encoded by this sequence naturally occurs in Elizabethkingia meningoseptica (syn.: Chryseobacterium meningosepticum and Flavobacterium meningosepticum). This sequence was optimized for codon usage in E. coli K12 and obtained by in-vitro gene synthesis.

References

The amino acid sequence of the protein is based on data from a publication in [http://www.nature.com/nbt/journal/v25/n4/abs/nbt1298.html Nature biotechnology]

Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464.

The sequence can be found in [http://www.ncbi.nlm.nih.gov/protein/205750037?report=genbank&log$=protalign&blast_rank=1&RID=3644MZXS01R NCBI].

A patent claiming rights on this part can be found in the [http://appft1.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=/netahtml/PTO/srchnum.html&r=1&f=G&l=50&s1=20140220553.PGNR. United States Patent & Trademark Office]