Regulatory

Part:BBa_K1424003

Designed by: Jacob Lamb   Group: iGEM14_NTNU_Trondheim   (2014-09-25)
Revision as of 09:46, 14 October 2014 by Lamja264 (Talk | contribs)

Kanamycin resistance for Synechocystis

The kanamycin resistance gene tn903 aph from E. coli for use with Synechocystis sp. PCC 6803.

The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006).

Conformation.jpg

Usage and Biology

ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to select positive integrants in the Synechocystis sp. PCC 6803 genome.

Improved Function

This part is an improvement to the documented part BBa_K300989. It has been optimized for use in the organism Synechocystis sp. PCC 6803, and also optimized to be compatible with iGEM assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None