Composite

Part:BBa_K1520017

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)
Revision as of 05:06, 14 October 2014 by Sagyman (Talk | contribs)

Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter

Plasmid1 in detector: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter.

It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp. WithoutAHL.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
biology
controlluxR, HSL
directionForward
functionRep
ligandsallolactose, IPTG
n/aconstitutive promoter family member
negative_regulators
o_h
o_l
positive_regulators1
proteinLacI
swissproP03023
tagLVA