Composite

Part:BBa_K1520018

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)
Revision as of 04:56, 14 October 2014 by Sagyman (Talk | contribs)

Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter

Plasmid2 in detector: Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter It is a low level detection plasmid. It suppresses the expression of lacI when AHL is enough, which will result in the expression of rfp when AHL is above a certain level.

AHLbelowT.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2223
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
controllambda cI
directionForward
functionRep
ligandsallolactose, IPTG
negative_regulators1
o_h
o_l
positive_regulators
proteinLacI
swissproP03023
tagLVA