Inverter

Part:BBa_K1385001

Designed by: Benjamin Huang   Group: iGEM14_WashU_StLouis   (2014-10-07)
Revision as of 23:07, 13 October 2014 by Benjaminhuang (Talk | contribs)

Hybrid CpcG2 promoter -> TetR

CpcG2 hybrid promoter. Maintains Upstream region of promoter with G-box, the binding site of CcaR to the promoter. A Phosphorylated CcaR binds to the G-box and transcribes the cpcg2 gene. G-box is a conserved sequence located -108 upstream of TSS

In this case, the g-box works as an activator for the light sensor mechanism. Replaced downstream (including -35 and -10 regions) with TetR


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 281
    Illegal NheI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
Parameters
None