Part:BBa_K1463000

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

Fig. 1: 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.

The image below shows a schematic of the switch. The BamHI sites are placed in such a way that the bands produced by digestion with this restriction enzyme change depending on whether recombination has occurred or not. This pattern can be seen on the gel above.

Sizes of BamHI fragments are: RFP ON GFP OFF (attP and attB) 2339bp + 2776bp GFP ON RFP OFF (attL and attR) 2628bp + 2477bp

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 813
  Illegal NheI site found at 836
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 793
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 34
  Illegal AgeI site found at 146
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 711
  Illegal BsaI.rc site found at 1668