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Sample In stock
Experience: Works
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Inverter

Part:BBa_K1385000

Designed by: Benjamin Huang   Group: iGEM14_WashU_StLouis   (2014-10-07)
Revision as of 17:56, 13 October 2014 by Benjaminhuang (Talk | contribs)

CPCG2 promoter -> TetR

This part incorporates the CpcG2 promoter from Jeff Tabor's 2010 paper "Multichromatic Control of Gene Expression in Escherichia coli" and makes it an inverter. This light regulated promoter expresses TetR in the presence of light. This part should be used in conjunction with https://parts.igem.org/Part:BBa_K1017726 to activate the light regulated plasmid, and a reporter plasmid such as https://parts.igem.org/Part:BBa_J70311 which is a pTet promoter a reporter protein EYFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 279
    Illegal NheI site found at 302
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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