Generator
Part:BBa_K1486002
Designed by: iGem EPFL 2014 Group: iGEM14_EPF_Lausanne (2014-08-12)
No part name specified with partinfo tag.
Purpose of the Unit
This unit (in the control layer) is a button used to apply pressure on the cells in the chamber. It was designed to decrease the outflow of cells into the channels.
Associated Biobricks
In the context of the same experiment, we designed another construct with the sfGFP moiety attached to the C terminus of CpxR: https://parts.igem.org/Part:BBa_K1486005.
Functional Parameters
[edit]
Categories
Parameters
//cds/reporter/gfp
//cds/transcriptionalregulator
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//classic/regulatory/other
//direction/forward
//function/regulation/transcriptional
//function/reporter/fluorescence
//promoter
//rbs/prokaryote/constitutive
//regulation/multiple
regulator
transcriptional
//cds/transcriptionalregulator
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//classic/regulatory/other
//direction/forward
//function/regulation/transcriptional
//function/reporter/fluorescence
//promoter
//rbs/prokaryote/constitutive
//regulation/multiple
regulator
transcriptional
biology | |
chassis | Escherichia coli |
color | Green |
control | AraC, arabinose |
direction | Forward |
emission | 510 |
excitation | 485 |
function | Transciption regulator |
negative_regulators | CpxA (phosphatase activity) |
positive_regulators | CpxA (Histidine kinase activity when activated) |
rbs | Elowitz |
resistance | Chloramphenicol |