Part:BBa_K1529300

Prhl(RL)

We newly developed the Prhl(RL) promoter (BBa_K1529300) which is activated by C4HSL (Fig. 1).
To characterize the function of this Prhl(RL) promoter, we constructed a part, Prhl(RL)-GFP (BBa_K1529301), by inserting the Prhl(RL) promoter, upstream of a GFP coding sequence.

Fig. 1. Our newly designed promoter

Fig. 2. Difference between LuxR and RhlR

By using the reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2).
We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay (Fig. 2).

Fig. 3. Fluorescence intensity detected by flow cytometer

For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]