Coding
Part:BBa_K1321012:Design
Designed by: Xenia Spencer-Milnes Group: iGEM14_Imperial (2014-10-10)
fMT metallothionein in Freiburg format (RFC[25])
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DNA sequence from BBa_K190019 was codon optimised using the propriety software from GeneArt®. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
Source
The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).