Coding
COMPx

Part:BBa_K1492000:Design

Designed by: Wiggert Altenburg   Group: iGEM14_TU_Eindhoven   (2014-07-28)
Revision as of 13:59, 13 October 2014 by Waltenburg (Talk | contribs) (→‎Design Notes)


Clickable Outer Membrane Protein x


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To integrate a Non-natural amino acid in the sequence of CPX some modifications were made. Within the part in which originally random mutations were induced a codon was mutated into the amber stop codon TAG. With a specific tRNA it is possible to implement a Non-Natural amino acid at the place of the TAG codon. The TAG codon was introduced into the Peptide Library by Site-Directed Mutagenesis. A HA-tag was also added to the C-terminus of the protein using overhang primers to use in characterization. After the modifications the protein was renamed to Clickable Outer Membrane Protein X (COMPx).

BBa k1492000 fig2.jpg750px|
Figure 2 Gene design of COMPx

Source

Outer Membrane Protein X. More information see Protein Data Bank or http://www.addgene.org/23336/

References