Conjugation
OriTRP4

Part:BBa_K1439000:Experience

Designed by: Wenqi Li   Group: iGEM14_OUC-China   (2014-09-28)
Revision as of 13:10, 13 October 2014 by Tifalee (Talk | contribs) (→‎Applications of BBa_K1439000)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1439000

We have test the oriTRP4 BBa_K1439000 in a conjugation system in which pSB1C3 with the help of plasmid RP4, and E. coli HB101 as donor and E.coil Top10 as recipient cells. We test the conjugative competence of oriTRP4 BBa_K1439000 by conjugate between HB101 and Top10. We use the RFP (BBa_J04450) marks the plasmids containing oriTRP4 BBa_K1439000. We get HB101 without streptomycin resistance form Zhigang Qiu. Top 10 has streptomycin resistance. After conjugation, we select recipient cells by streptomycin resistance LB medium and statistical number of red bacterial colony.

User Reviews

UNIQ8101bc8c50bd8e86-partinfo-00000000-QINU UNIQ8101bc8c50bd8e86-partinfo-00000001-QINU Test

Conjugate between E.coil HB101 and E.coil Top10.