Composite

Part:BBa_K1520514

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-23)
Revision as of 20:21, 12 October 2014 by Sagyman (Talk | contribs)

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MC31-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

MC31-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our putative suicide circuit for lacking microcystin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2853
    Illegal NheI site found at 2876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4054
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
biology
controlaTc, tetracyline
directionForward
functionRep
ligandstetracycline
n/aconstitutive promoter family member
negative_regulators1
o_h
o_l
positive_regulators
proteinTetR
swissproP04483
tagLVA