Coding

Part:BBa_K1420001

Designed by: Stephen C. Heinsch   Group: iGEM14_Minnesota   (2014-09-26)
Revision as of 19:06, 12 October 2014 by Skog0122 (Talk | contribs)

merA, mercuric reductase from Serratia marcescens

Function

Mercury resistant genemerA encodes a a mercuric ion reductase (MerA) that catalyze the reduction of mercuric ion(Hg2+) to the relative inert, volatile monoatomic mercury (Hg0) in a NADPH dependent reaction. While orchestrating with the transportation system composed of other gene products of Mer Operon, MerA and MerB are the key detoxification enzymes that confer mercury resistance. Scheme 1. MerA Catalyzed Reaction <p>

File:MerA reaction Mechanism 

 </p>

Structure and Mechanism

Zone of Inhibition Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
    Illegal NgoMIV site found at 1249
    Illegal NgoMIV site found at 1311
    Illegal NgoMIV site found at 1522
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds
Parameters
None