Coding

Part:BBa_K1321337

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 15:50, 12 October 2014 by Xen sm (Talk | contribs)

sfGFP in Freiburg format (RFC 25)

sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.

Usage and Biology

Superfolder Green Fluorescent Protein (sfGFP) is a mutant of mut3b GFP (E0040) with improved in vivo properties. It was first entered into the registry by the Cambridge iGEM team in 2008; please see I746916 part description and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP for detailed information about the properties of sfGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


[edit]
Categories
//cds/reporter/gfp
//function/reporter/fluorescence
Parameters
colorGreen