Coding

Part:BBa_K1403019

Designed by: Paris Bettencourt 2014   Group: iGEM14_Paris_Bettencourt   (2014-10-06)
Revision as of 14:48, 12 October 2014 by Ysylvia (Talk | contribs)

Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette

ilvBN CDS from E. coli MG1655

ilvBN gene exists in natural wild type E.Coli strain. They codes for acetolactate synthase process. Here, we isolated it from MG1655 strain through PCR to transform it into targeted strain for increased basal production of acetolactate, which serves as precursor for acetoin production.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None