Generator

Part:BBa_K1403003:Design

Designed by: Sylvia   Group: iGEM14_aris_Bettencourt   (2014-10-06)
Revision as of 13:47, 12 October 2014 by Ysylvia (Talk | contribs) (→‎Design Notes)

Limonene synthase (LIMS1) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.

We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format.

Source

Registry. BBa_I742110

References

Edinburg iGEM Project 2007